Fig. 3

Premature differentiation and aberrant cell death of Trk-positive neuronal subtypes in the DRG of Wnt1-Cre; Mib1^fl/fl embryos (a–f). Transverse serial sections over the forelimb of wildtype and Wnt1-Cre; Mib1^fl/fl embryos at E10.5 co-immunostained for the neuronal marker Isl1, cell death marker cleaved-Caspase 3 (Casp3), and the neurotrophin receptors TrkA (a, b), TrkB (c, d) and TrkC (e, f). Scale bar = 20 μm. g Quantitation of Trk and Isl1-double-positive cell bodies per DRG at E10.5. Number represents mean of n = 3 embryos, with 8 individual sections counted per embryo. *p < 0.05, **p < 0.01, ****p < 0.0001. h–m Transverse serial sections over the forelimb of wildtype and Wnt1-Cre; Mib1^fl/fl embryos at E11.5 co-immunostained for Isl1, Casp3, and TrkA (h, i), TrkB (j, k) and TrkC (l, m). Scale bar = 20 μm. n Quantitation of Trk and Isl1-double-positive cell bodies per DRG at E11.5. Number represents mean of n = 3 embryos, with 8 individual sections counted per embryo, ns not significant, *p < 0.05, ****p < 0.0001