Table 1 The premier sequences
Factor | Forward | Reverse | Annealing temperature (°C) |
|---|---|---|---|
β-actin | 5′GAAGATCAAGATCATTGCTCC3′ | 5′TACTCCTGCTTGCTGATCCA3′ | 52 |
COX5A | 5′GTTGGACCAATCATAGGCGCT3′ | 5′CAATGTCGATCACATGCACCA3′ | 56 |
Rho-GDIa | 5′ TACAAGCCTCCAGCCCAGAA 3′ | 5′ AAGGTCAAGCGGGTCACAAT3′ | 53 |
Gstp1 | 5′ TCAAGGCTCGCTCAAGTCCA 3′ | 5′ TTGCATCGAAGGTCCTCCAC 3′ | 52 |
Sod2 | 5′ CCCTGACCTGCCTTACGACT 3′ | 5′ AGCGACCTTGCTCCTTATTG 3′ | 53 |
TPI | 5′ GGGGCAACTGGAAGATGAA 3′ | 5′ CCTGGCGAAGTCGATGTAG 3′ | 52 |