Fig. 2

The effect of tooth movement on orofacial pain level and N/OFQ expression. The rats were respectively treated with the force of 0 g, 20 g, 40 g and 80 g (n = 30 for each group, n = 5 for each group each day). a Temporal changes of RGS scores in all the force groups. Two-way ANOVA with repeated measures were used to analyze the effects of force magnitudes, time and their interactions on the RGS scores. One-way ANOVA was used to analyze RGS scores in different force groups at different timepoint (*p < 0.05, **p < 0.01, 0 g group vs. 20 g group vs. 40 g group vs. 80 g group). b One-way ANOVA with Bonferroni post hoc test was used to analyze the area under curve (AUC) of RGS scores (*p < 0.05, 40 g group vs. 0 g group, 80 g group vs. 0 g group). c Western blot analysis for the quantification of N/OFQ expression in trigeminal ganglia, with β-actin being the internal reference. The images were cropped using Adobe Photoshop software, and full-length blots/gels are presented in Additional file 3: Figure S1. d Temporal changes of N/OFQ expression in all the force groups. Two-way ANOVA with repeated measures were used to analyze the effects of force magnitudes, time and their interactions on the expression levels of N/OFQ. One-way ANOVA was used to analyze N/OFQ expression levels in different force groups at the same timepoint (*p < 0.05, **p < 0.01, 0 g group vs. 20 g group vs. 40 g group vs. 80 g group). e One-way ANOVA with Bonferroni post hoc test was used to analyze the AUC of the expression level of N/OFQ (*P < 0.05, 40 g group vs. 0 g group, 80 g group vs. 0 g group)