Fig. 3

Cutaneous inflammation and mast cell hypertrophy precedes brain inflammation in VPA-exposed mice. A, B Dense cutaneous inflammatory infiltrate by day 1 in VPA-exposed mice (H + E staining). C, D Mast cell hypertrophy and degranulation at day 1 in toluidine blue-stained, one µm sections E Quantitation of mast cell density in VPA-exposed skin at days 1 and 4. F Changes in circulating IgE levels over time