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Fig. 5 | BMC Neuroscience

Fig. 5

From: Albumin alleviated esketamine-induced neuronal apoptosis of rat retina through downregulation of Zn2+-dependent matrix metalloproteinase 9 during the early development

Fig. 5

Effect of albumin on MMP9 expression and enzyme activity in GCL. A Representative images show MMP9 histoimmunostaining in retinal. Scale bar, 100 μm. B Bar graph shows the shows the fluorescence intensity of MMP9 expression (n = 4 retinas per group). C Representative images show MMP9 activity in situ zymography staining in GCL. Scale bar, 50 μm. D Bar graph shows the fluorescence intensity of MMP9 activity in GCL (n = 4 retinas per group). E and F Representative images of western blot and bar graph summary show pro MMP9 (92 kDa) and active MMP9 protein (68 kDa) expression in whole retina tissue (n = 3 retina per group). The full-length blots are presented in Additional file 2: Figure S1. * P < 0.05, *** P < 0.001 compared with control group; † P < 0.05, ††† P < 0.001 compared with esketamine group

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