Fig. 2
Suppressive effects of varying doses of the selective 5-HT2A receptor antagonist EMD 281014 (0, 0.001, 0.005, 0.01 and 0.05 mg/kg, i.p.) on the mean (± SEM) frequency of HTR induced by d-fenfluramine (5 mg/kg, i.p.) in 20-, 30- and 60-day old mice. Among the EMD 281014 vehicle-pretreated control age groups, significant differences in the mean frequency of d-fenfluramine-induced HTR were observed between 20- and 30-day (####p < 0.0001) and between 30- and 60-day old mice (####p < 0.0001). Varying doses of EMD 281014 suppressed d-fenfluramine-induced HTR in a dose-dependent manner across the age-range tested. Compared to corresponding age-matched vehicle-pretreated control group, significant reductions were observed at all tested doses of EMD 281014 in 30-day old mice, but only at 0.005, 0.01 and 0.05 mg/kg doses in 20- and 60-day old mice. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. vehicle injection; two-way ANOVA followed by Dunnett's test. n = 5 − 9 in each group. Data are presented as means ± SEM