Fig. 7

Effects of MA on d-fenfluramine-induced c-fos expression in different regions at 5 coronal sections in the PFC of mice. Relative to Vehicle + Vehicle group, d-fenfluramine by itself (i.e., Vehicle + d-fenfluramine) significantly increased c-fos expression in the areas in mice PFC as described in Fig. 6. Compared to the Vehicle + Vehicle control group, MA by itself (i.e. MA + Vehicle group) significantly increased c-fos expressions in the: (1) FrA, PrL, LO and DLO at the level of − 2.68 mm relative to bregma (a, b); (2) M1, M2, Cg1 and AI at the level of − 2.34 and − 2.1 mm relative to bregma (c–f); (3) S1 and M1 at the level of − 1.98 and − 1.7 mm relative to bregma (g, i); inclusion of d-fenfluramine (i.e. MA + d-fenfluramine treatment group) significantly increased c-fos expression in the: (1) FrA, PrL, LO and DLO at the level of − 2.68 mm relative to bregma (a, b); (2) M1, M2 and AI at the level of − 2.34 and − 2.1 mm relative to bregma (c–f); (3) S1, M1 and AI at the level of − 1.98 mm relative to bregma (g, h); (4) S1, M1 and M2 at the level of − 1.7 mm relative to bregma (i). Compared to the Vehicle + d-fenfluramine group, pre-treatment with MA (i.e. MA + d-fenfluramine group) significantly increased c-fos expression in the: (1) FrA, LO and DLO at the level of − 2.68 mm relative to bregma (a, b); (2) M2 at the level of − 2.34 mm relative to bregma (c); (3) M1, M2 and AI at the level of − 2.1 mm relative to bregma (e, f); (4) S1 and M1 at the level of − 1.98 and − 1.7 mm relative to bregma (g, i). Neither MA alone nor in combination with d-fenfluramine produced any significant changes in c-fos expression in the: (1) MO, VO at the level of − 2.68 mm relative to bregma (a, b); (2) PrL, MO, VO and LO at the level of − 2.34 and − 2.1 mm relative to bregma (c–f); (3) M2, Cg1, PrL, IL, MO, VO and LO at the level of − 1.98 mm relative to bregma (g, h); and (4) Cg1, PrL, IL and DP at the level of − 1.7 mm relative to bregma (i, j). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Vehicle + Vehicle group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 vs. Vehicle + d-fenfluramine group; one-way ANOVA followed by Tukey's test. Data are presented as means ± SEM